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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Investigation of Kluyveromyces marxianus as a novel host for large‐scale production of porcine parvovirus virus‐like particles

Fig. 5

pH value effects on the cation exchange purification of PPV VLPs with Capto S ImpAct resin. The KM-PPV-VP2 cell lysates disrupted with PBS buffer pH7.4, and adjusted to pH 5.3–4.0 with acetic acid. After centrifugation at 12,000 rpm for 15 min, 2 ml supernatants were loaded onto Biorad Poly-Prep chromatography columns packaged with 1 mL Capto S ImpAct resins and eluted PBS pH7.4 containing 0.5 M NaCl. Lane F: flow-through fractions; Lane E: elution fractions

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Microbial Cell Factories

ISSN: 1475-2859

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