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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Investigation of Kluyveromyces marxianus as a novel host for large‐scale production of porcine parvovirus virus‐like particles

Fig. 1

Expression and characterization of the PPV VP2 protein in K. marxianus. a Illustration of the recombinant expression plasmid pUKDN115-PPV VP2. The inulinase promoter, inulinase terminator, and URA3 cassette in pUKDN115 are all derived from K. marxianus, and autonomously replicating sequence is from pKD1. b TEM scan of the assembled PPV VLPs in K. marxianus intracellularly. SDS-PAGE (c) and Western Blot (b) assays of the lysates of recombinant strain after shaked for 66 h. Lane M: PageRuler Prestained Protein Ladder;Lane 1: the lysate of Fim-1 ∆ura3 transformed with the empty plasmid pUKDN115; Lane 2: the lysate of KM-PPV-VP2; Lane 3: the supernatant of KM-PPV-VP2 cell lysate; Lane 4: the precipitate of KM-PPV-VP2 cell lysate

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Microbial Cell Factories

ISSN: 1475-2859

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