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Fig. 8 | Microbial Cell Factories

Fig. 8

From: Efficient whole-cell oxidation of α,β-unsaturated alcohols to α,β-unsaturated aldehydes through the cascade biocatalysis of alcohol dehydrogenase, NADPH oxidase and hemoglobin

Fig. 8

Time courses of alcohol oxidation at higher substrate concentrations. The reaction mixture (5 ml) contained 50–300 mM 3-methyl-2-buten-1-ol, 0.3 g lyophilized cells, 0.4 mM NADP+, 0.2 mM FAD, and 50 mM Tris-HCl (pH 8.0). The reaction was carried out at 45 °C and 600 rpm for 2 h. Standard deviations are indicated in the diagram (n = 3)

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