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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Efficient whole-cell oxidation of α,β-unsaturated alcohols to α,β-unsaturated aldehydes through the cascade biocatalysis of alcohol dehydrogenase, NADPH oxidase and hemoglobin

Fig. 7

Effect of FAD and NADP+ coenzymes on the catalytic performance of cells expressing the YsADH-(GSG)-TkNOX-(GSG)-VsHGB fusion enzyme. The reaction mixture (5 ml) contained 100 mM 3-methyl-2-buten-1-ol, 0.1 g lyophilized cells, 0–1.0 mM NADP+, 0–1.0 mM FAD, and 50 mM Tris-HCl (pH 8.0). The effect of the FAD concentration was investigated at an NADP+ concentration of 0.2 mM, and that effect of the NADP+ concentration was then evaluated at an FAD concentration of 0.2 mM. All the reactions were carried out at 45 °C and 600 rpm for 2 h. Standard deviations are indicated in the diagram (n = 3)

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Microbial Cell Factories

ISSN: 1475-2859

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