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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Efficient whole-cell oxidation of α,β-unsaturated alcohols to α,β-unsaturated aldehydes through the cascade biocatalysis of alcohol dehydrogenase, NADPH oxidase and hemoglobin

Fig. 5

Effect of pH on the catalytic performance of cells expressing the fusion enzyme YsADH-(GSG)-TkNOX-(GSG)-VsHGB. The reaction mixture (5 ml) contained 100 mM 3-methyl-2-buten-1-ol, 0.1 g lyophilized cells, 0.4 mM NADP+, 0.2 mM FAD, 50 mM buffer (PIPES, pH 6.0, 6.5 or 7.0; Tris-HCl, pH 7.5, 8.0, 8.5 or 9.0; CAPS, pH 9.5). The reactions were carried out at 45 °C and 600 rpm for 2 h. Standard deviations are indicated in the diagram (n = 3)

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Microbial Cell Factories

ISSN: 1475-2859

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