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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Efficient whole-cell oxidation of α,β-unsaturated alcohols to α,β-unsaturated aldehydes through the cascade biocatalysis of alcohol dehydrogenase, NADPH oxidase and hemoglobin

Fig. 4

Effect of temperature on the catalytic performance of cells expressing the fusion enzyme YsADH-(GSG)-TkNOX-(GSG)-VsHGB. The reaction mixture (5 ml) contained 100 mM 3-methyl-2-buten-1-ol, 0.1 g lyophilized cells, 0.4 mM NADP+, 0.2 mM FAD, and 50 mM Tris-HCl (pH 8.0). The reactions were carried out at 40–65 °C and 600 rpm for 2 h. Standard deviations are indicated in the diagram (n = 3)

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