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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Efficient whole-cell oxidation of α,β-unsaturated alcohols to α,β-unsaturated aldehydes through the cascade biocatalysis of alcohol dehydrogenase, NADPH oxidase and hemoglobin

Fig. 3

Effect of the linker type on the catalytic performance of the fusion enzyme YsADH-(linker)-TkNOX. The control represents the E. coli cells coexpressing YsADH, TkNOX and VsHGB separately. The reaction mixture (5 ml) contained 100 mM 3-methyl-2-buten-1-ol, 0.3 g lyophilized cells, 0.4 mM NADP+, 0.2 mM FAD, and 50 mM Tris-HCl (pH 8.0). The reaction was carried out at 45 °C and 600 rpm for 2 h. Standard deviations are indicated in the diagram (n = 3)

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Microbial Cell Factories

ISSN: 1475-2859

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