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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Efficient whole-cell oxidation of α,β-unsaturated alcohols to α,β-unsaturated aldehydes through the cascade biocatalysis of alcohol dehydrogenase, NADPH oxidase and hemoglobin

Fig. 2

SDS-PAGE analysis of the YsADH-(linker)-TkNOX and YsADH-(linker)-TkNOX-(linker)-VsHGB fusion proteins in cell-free extracts. Lane M, marker; lane 1, expression of YsADH-(GSG)-TkNOX-(GSG)-VsHGB (99.7 kDa); lane 2, expression of YsADH-(GSG)-TkNOX (80.7 kDa); lane 3, expression of YsADH-(GSG)2-TkNOX (80.7 kDa); lane 4, expression of YsADH-(GGGGS)-TkNOX (80.7 kDa); lane 5, expression of YsADH-(GGGGS)2-TkNOX (80.7 kDa); lane 6, expression of TkNOX (42.7 kDa); lane 7, expression of YsADH (32.7 kDa); lane 8, uninduced cells coexpressing YsADH, TkNOX and VsHGB as the control. The percentage of acrylamide in the resolving gel was 12%. The value in the bracket represents the apparent molecular mass of the fusion enzyme, YsADH or TkNOX

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Microbial Cell Factories

ISSN: 1475-2859

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