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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Engineering Escherichia coli for the utilization of ethylene glycol

Fig. 7

Fermentation profiles for aeration strategies. Two different aeration strategies were tested to improve glycolate production. The growth phase and production phase are separated by grey shading. a The first strategy employed a high flow rate of 600 mL/min during the growth phase, and a flow rate of 100 mL/min during the production phase. The final growth phase cell density was approximately 4 gDW/L and the final glycolate titer reached 6.8 g/L. b The second strategy used a consistently low flow rate of 50 mL/min during both the growth and production phases, but the impeller speed was reduced during the production phase such that the respiratory quotient (RQ) was approximately 0.4. The average stationary phase cell density was 2.5 gDW/L. Cells were capable of robust glycolate production for well over 100 h in the production phase, reaching a final glycolate titer of 10.4 g/L

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