Protective effects of a food-grade recombinant Lactobacillus plantarum with surface displayed AMA1 and EtMIC2 proteins of Eimeria tenella in broiler chickens

Table 1 Stability of erm-marked and asd-alr-marked plasmids

Plasmid/medium	50 generations	100 generations
Plasmid/medium	Percentage containing plasmid	Percentage containing plasmid
pLP-pgsA′-EGFP/MRS	46	0
pLP-EGFP-S/MRS	48	0
pLQa-pgsA′-EGFP/MRS	100	100
pLQa-EGFP-S/MRS	100	100
pLp-pgsA′-EGFP/MRS + erythromycin	91	83
pLp-EGFP-S/MRS + erythromycin	93	86
pLQa-pgsA′-EGFP/MRS + d-ala	83	66
pLQa-EGFP-S/MRS + d-ala	85	69

^aL. plantarum NC8 containing pLp-pgsA′-EGFP and pLP-EGFP-S, respectively, were cultivated in MRS solid media with or without 5 μg/mL erythromycin
^bL. plantarum NC8/Δalr containing pLQa-pgsA′-EGFP and pLQa-EGFP-S, respectively, were cultivated in MRS solid media with or without 200 μg/mL d-ala. These strains were sub-cultivated at 30 °C without agitation and passaged every 12 h. The percentage containing plasmid was calculated as the number of single colonies on selected plates

ISSN: 1475-2859