Fig. 7From: High copy number and highly stable Escherichia coli–Bacillus subtilis shuttle plasmids based on pWB980The 10 L fermentations of WB600 (pUC980-2-pelN). a The cell wet-weights, OD600nm and the pectate lyase activities were determined for every 6 h. Cells went into the stationary phase after 30-hours fermentation and the cell wet-weight was 66.3 g/mL with OD600nm of 34.1. The highest activity were obtained at 54 h with 5200 U/mL. Then the enzyme productions decreased gradually. b The fermentations of WB600 (pUC980-2-pelN) have been repeated for 20 times and the PelN activities kept stable and repeatableBack to article page