Fig. 6From: High copy number and highly stable Escherichia coli–Bacillus subtilis shuttle plasmids based on pWB980Pullulanase productions in B. subtilis WB600. a Activities of pullulanase expressed by different plasmids were determined in supernatants of WB600. The highest activity was obtained by pUC980-2 with 187 U/mL. b SDS-PAGE analyses of pullulanase expressions, M was the protein molecular weight marker. The PulA11 protein was 81.7 kDa as shown in the frameBack to article page