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Fig. 4 | Microbial Cell Factories

Fig. 4

From: High copy number and highly stable Escherichia coliBacillus subtilis shuttle plasmids based on pWB980

Fig. 4

Alkaline pectate lyase productions in B. subtilis. a All plasmids were used to express alkaline pectate lyase pelN in B. subtilis WB600. The highest activity was obtained by pUC980-2 with 2738 U/mL. b SDS-PAGE analysis of PelN in culture supernatants. Lane M was protein molecular weight marker. The PelN protein was 48.0 kDa as shown in the frame. Lane 1 was the starting strain WB600, served as negative control. Lanes 2-10 were WB600 with plasmids pWB980, pWB980-DB and pUC980-serial plasmids (pUC980-1 to pUC980-7). Lanes 11-19 represented WB600 with PelN-expressing plasmids pWB980-pelN, pWB980-DB-pelN and pUC980-pelN plasmids (pUC980-1-pelN to pUC980-7-pelN) correspondingly

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