Fig. 4
The effects of tHMG1 overexpression and dynamic downregulation of ERG9 on the production of (+)-valencene in different metabolically engineered S. cerevisiae strains. tHMG1 was overexpressed in strains V02, V04, V05, and V06, resulting in strains V09, V10, V11, and V13, respectively. The production of (+)-valencene was determined by GC-FID analysis. The ERG9 was further downregulated in strain V11 and V13 by replacing its endogenous promoter with PHXT1 promoter, resulting in V15 and V16 strains, respectively. The production of (+)-valencene was determined by GC-FID analysis