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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Identification of a Yarrowia lipolytica acetamidase and its use as a yeast genetic marker

Fig. 2

Gene deletion confirmation. Genomic DNA from strains YB-392, NS995 and NS996 was probed for the presence of the deletion target genes (“wt”) and the integrated antibiotic resistance cassette (“nat”) by PCR. YB-392 contains both the YlAMD1 gene YALI0E34771 (lane 3) and YALI0E11847 (lane 7) but neither nat1 integration-specific product (lanes 2 and 6). PCR confirms the absence of YALI0E34771 and YALI0E11847 in NS995 and NS996 respectively (lanes 5 and 9) while each of these strains contains the targeted-integration specific nat1 product (lanes 4 and 8)

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