Table 1 Strains and plasmids used in this study

Strains

 TOP10

F⁻mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Str^R) endA1 nupG

Invitrogen

 BL21 (DE3)

F⁻ompT gal dcm lon hsdS_B(r⁻_Bm⁻_B) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB⁺]_K-12(λ^S)

NEB

 MG1655

F⁻, λ⁻, rph-1

KCTC

 MGI

MG1655△ilvG::dxs△glvC::idi△yjbI::ispA△agaAV::dxr

unpublished

 MGIG

MGI△ldhA::GGPPS

This study

 MGIUG

MGI△ldhA::UTR GGPPS

This study

 MGIUK

MGIUE△malT::UTRCDPSKS

This study

 MGIUKN

MGIUK△gdhA

This study

Plasmids

 pUCM

Cloning vector derived from pUC19; constitutive lac promoter, Amp^R

[25]

 pUCM_GGPPS

ColE1 ori, constitutive lac promoter, expressing GGPPS, Amp^R

[5]

 pUCM_CDPS

ColE1 ori, constitutive lac promoter, expressing CDPS, Amp^R

[5]

 pUCM_KS

ColE1 ori, constitutive lac promoter, expressing KS, Amp^R

[5]

 pUCM_GGPPS(U)

ColE1 ori, constitutive lac promoter, expressing GGPPS, synthetic 5′-UTR, Amp^R

This study

 pUCM_CDPS(U)

ColE1 ori, constitutive lac promoter, expressing CDPS, synthetic 5′-UTR, Amp^R

This study

 pUCM_KS(U)

ColE1 ori, constitutive lac promoter, expressing KS, synthetic 5′-UTR, Amp^R

This study

 pSTVM2

Cloning vector derived from pSTV29; constitutive lac promoter, Cm^R

This study

 pSTVM_CK

p15A ori, constitutive lac promoter, expressing CDPS and KS, Cm^R

This study

 pSTVM_GCK

p15A ori, constitutive lac promoter, expressing GGPPS, CDPS, and KS, Cm^R

This study

 pSTVM_GCK(U)

p15A ori, constitutive lac promoter, expressing GGPPS, CDPS and KS, synthetic 5′-UTR, Cm^R

This study

 pUCrop

ColE1 ori, rop, Amp^R

This study

 pUCrop_AtKO

ColE1 ori, rop, constitutive lac promoter, expressing AtKO, Amp^R

This study

 pUCrop_AtCPR2

ColE1 ori, rop, constitutive lac promoter, expressing AtCPR2, Amp^R

This study

 pUCrop_trAtKO

ColE1 ori, rop, constitutive lac promoter, expressing modified AtKO, Amp^R

This study

 pUCrop_trAtKO (U)

ColE1 ori, rop, constitutive lac promoter, expressing modified AtKO, synthetic 5′-UTR, Amp^R

This study

 pUCrop_AtKO_AtCPR2

ColE1 ori, rop, constitutive lac promoter, expressing AtKO and AtCPR2, Amp^R

This study

 pUCrop_trAtKO_AtCPR2

ColE1 ori, rop, constitutive lac promoter, expressing modified AtKO and AtCPR2, Amp^R

This study

 pUCrop_SrKO_AtCPR2

ColE1 ori, rop, constitutive lac promoter, expressing SrKO and AtCPR2, Amp^R

This study

 pUCrop_trSrKO_AtCPR2

ColE1 ori, rop, constitutive lac promoter, expressing modified SrKO and AtCPR2, Amp^R

This study

 pUCrop_trAtKO(U)_AtCPR2

ColE1 ori, rop, constitutive lac promoter, expressing modified AtKO and AtCPR2, synthetic 5′-UTR, Amp^R

This study

 pUCrop_trCYP714A2(U)

ColE1 ori, rop, constitutive lac promoter, expressing modified CYP714A2, synthetic 5′-UTR, Amp^R

This study

 pUCrop_Fusion5(U)

ColE1 ori, rop, constitutive lac promoter, expressing modified CYP714A2 and AtCPR2, chimera, synthetic 5′-UTR, Amp^R

This study

 pUCrop_Fusion10(U)

ColE1 ori, rop, constitutive lac promoter, expressing modified CYP714A2 and AtCPR2, chimera, synthetic 5′-UTR, Amp^R

This study

 pUCrop_Fusion15(U)

ColE1 ori, rop, constitutive lac promoter, expressing modified CYP714A2 and AtCPR2, chimera, synthetic 5′-UTR, Amp^R

This study

 pUCrop_trAtKO(U)_AtCPR2_trCYP714A2(U)

ColE1 ori, rop, constitutive lac promoter, expressing modified AtKO, AtCPR2 and modified CYP714A2, synthetic 5′-UTR, Amp^R

This study

 pUCrop_trAtKO(U)_AtCPR2_Fusion5(U)

ColE1 ori, rop, constitutive lac promoter, expressing modified AtKO, AtCPR2 and fusion protein, synthetic 5′-UTR, Amp^R

This study

 pUCrop_trAtKO(U)_AtCPR2_Fusion10(U)

ColE1 ori, rop, constitutive lac promoter, expressing modified AtKO, AtCPR2 and fusion protein, synthetic 5′-UTR, Amp^R

This study

 pUCrop_trAtKO(U)_AtCPR2_Fusion15(U)

ColE1 ori, rop, constitutive lac promoter, expressing modified AtKO, AtCPR2 and fusion protein, synthetic 5′-UTR, Amp^R

This study

 pET21α(+)

f1 ori, T7 promoter, C-terminal His-tag sequence, Amp^R

Novagen

 pET21α(+)_AtKO

f1 ori, T7 promoter, inducible expression of His₆-tagged AtKO, Amp^R

This study

 pET21α(+)_trAtKO

f1 ori, T7 promoter, inducible expression of His₆-tagged modified AtKO, Amp^R

This study

 pMP11

pKD46 with constitutively expressed Cas9, aTc gRNA targeting ColE1 origin, Amp^R

[27]

 pgRNA

Constitutively expressed sgRNA

[27]

 pgRNA_ldhA

Constitutively expressed sgRNA targeting ldhA, ColE1 ori, Cm^R

This study

 pgRNA_malT

Constitutively expressed sgRNA targeting gdhA, ColE1 ori, Cm^R

This study

 pgRNA_gdhA

Constitutively expressed sgRNA targeting malT, ColE1 ori, Cm^R

This study