Fig. 5

AmB production associated with genes overexpression and the fermentation time courses. a AmB production associated with genes overexpression in various engineered strains, the genetically engineered strains wereall constructed from primitive stain, S. nodosus ZJB2016050. ZJB2016050 represents strain S. nodosus ZJB2016050, pJTU1278 represents strain S. nodosus ZJB2016050 with plasmid pJTU1278. vhb, metK, amphRI, amphRIV, amphGH, amphG and araC represent overexpression of gene vhb, metK, amphRI, amphRIV, amphH, amphG and araC, respectively, in strain S. nodosus ZJB2016050 with plasmid pJTU1278. VMR4A and VMR4HGA were strain overexpressed four genes (vhb, metK, amphRIV and araC connected by ermE*p) and six genes (vhb, metK, amphRIV, amphH, amphG and araC connected by ermE*p), respectively. Samples were collected from soluble fermentation at 144 h, and the AmB concentration and ration of AmA were detected and analyzed, respectively. b Fermentation time course for strain S. nodosus ZJB2016050, pJTU1278, VMR4A and VMR4HGA. The ZJB2016050 and pJTU1278 were primitive strain and the strain with empty vector, respectively. VMR4A and VMR4HGA were strain overexpressed four genes (vhb, metK, amphRIV and araC connected by ermE*p) and six genes (vhb, metK, amphRIV, amphH, amphG and araC connected by ermE*p), respectively. Each value is a mean of three experiments. Error bars show standard derivation among three experiments. Symbol ‘*’ means the experimental strain compared with the original strain ZJB2016050 and ^× means the experimental strain compare with strain with vector pJTU1278 (*p < 0.05, **p < 0.01, ^×p < 0.05 and ^××p < 0.01)