Improving phytosterol biotransformation at low nitrogen levels by enhancing the methylcitrate cycle with transcriptional regulators PrpR and GlnR of Mycobacterium neoaurum

Table 2 Recombinant strain growth status and AD conversion ratio in different levels of nitrogen sources

Strains	N^0.1 (0.35 g/L NH₄₂PO₄)		N^0.5 (1.75 g/L NH₄₂PO₄)		N¹ (3.5 g/L NH₄₂PO₄)
Strains	Final OD₆₀₀	Max AD converted (%)	Final OD₆₀₀	Max AD converted (%)	Final OD₆₀₀	Max AD converted (%)
MNR	6.5 ± 0.2	13.8 ± 2.8	10.3 ± 0.3	42.4 ± 2.7	11.5 ± 0.6	75.7 ± 3.5
MNRΔglnR	7.0 ± 0.3	16.7 ± 2.3	10.8 ± 0.4	49.3 ± 2.3	10.8 ± 0.5	86.3 ± 3.7
MNR-prpDBC/ΔglnR	6.8 ± 0.2	17.3 ± 2.5	10.3 ± 0.3	52.3 ± 2.8	10.2 ± 0.5	91.7 ± 3.6
MNR-prpR/ΔglnR	6.7 ± 0.2	21.6 ± 2.4	10.8 ± 0.4	57.7 ± 2.8	11.2 ± 0.6	94.3 ± 3.4

ISSN: 1475-2859