Fig. 6

Functional analysis of KdFRS in yeast. a Comparison of extracted ion GC–MS chromatograms at m/z 426 (friedelin parent mass) of hexane extracts of the strains AM109-3 (KdFRS, blue), control strain AM109-1 (empty vector, black), and authentic friedelin standard (red), showing accumulation of friedelin in AM109-3. b The constituent eluting at 32 min retention time shows the same mass spectrum as the friedelin standard. c Friedelin accumulation in yeast strains engineered for terpenoid production. Total friedelin production in the strains AM109-3, AM238-2 and AM254-2 expressing KdFRS was quantified by LC–APCI–MS analysis of hexane extracts of saponified cell pellets. (n = 3, error bars correspond to standard deviation.)