Table 1 The primers used in this study
From: Homogeneous production and characterization of recombinant N-GlcNAc-protein in Pichia pastoris
Primers | Sequence |
|---|---|
MNS1-F | 5-CCGGAAGGCGCCACCATGAAGAACTCTGTCGGTATTTCAATTGCAACCATTGTTGCTATCATAGCAG-3 |
MNS1-R | 5-CCGCTCGAGTCTCTCAAAGTGTTCGTACCATGGCACATAGTATATAGCTGCTATGATAGCAACAATG-3 |
EndoT-F (EcoRI) | 5-CGGAATTCGTTCCTGTCAAGGAGTTGCA-3 |
EndoT-R-Pir (MluI) | 5-CGACGCGTTTACTTATCGTCATCGTCCT-3 |
EndoT-R (NotI) | 5-ATAAGAATGCGGCCGCTTACTTATCGTCATCGTCCT-3 |
GalNAc-T1-F (SnaBI) | 5-GACCTACGTAGGACTTCCTGCTGAAGATGT-3 |
GalNAc-T1-R (NotI) | 5-ATAAGAATGCGGCCGCTAGTGATGATGATGATGATGATGGAATATTTCTGGCAGGGTGAC-3 |
Fc-F (EcoRI) | 5-CCGGAATTCGAACCCAAGTCCTGCGAC-3 |
Fc-R (NotI) | 5-ATAAGAATGCGGCCGCTCACTTGCCGGGGCTCAG-3 |
pGAP-F | 5-GTCCCTATTTCAATCAATTGAA-3 |
5′AOX | 5-GACTGGTTCCAATTGACAAGC-3 |