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Table 1 The primers used in this study

From: Homogeneous production and characterization of recombinant N-GlcNAc-protein in Pichia pastoris

PrimersSequence
MNS1-F5-CCGGAAGGCGCCACCATGAAGAACTCTGTCGGTATTTCAATTGCAACCATTGTTGCTATCATAGCAG-3
MNS1-R5-CCGCTCGAGTCTCTCAAAGTGTTCGTACCATGGCACATAGTATATAGCTGCTATGATAGCAACAATG-3
EndoT-F (EcoRI)5-CGGAATTCGTTCCTGTCAAGGAGTTGCA-3
EndoT-R-Pir (MluI)5-CGACGCGTTTACTTATCGTCATCGTCCT-3
EndoT-R (NotI)5-ATAAGAATGCGGCCGCTTACTTATCGTCATCGTCCT-3
GalNAc-T1-F (SnaBI)5-GACCTACGTAGGACTTCCTGCTGAAGATGT-3
GalNAc-T1-R (NotI)5-ATAAGAATGCGGCCGCTAGTGATGATGATGATGATGATGGAATATTTCTGGCAGGGTGAC-3
Fc-F (EcoRI)5-CCGGAATTCGAACCCAAGTCCTGCGAC-3
Fc-R (NotI)5-ATAAGAATGCGGCCGCTCACTTGCCGGGGCTCAG-3
pGAP-F5-GTCCCTATTTCAATCAATTGAA-3
5′AOX5-GACTGGTTCCAATTGACAAGC-3