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Table 1 List of partial differential expression proteins identified by the iTRAQ analysis

From: Effects of ccpA gene deficiency in Lactobacillus delbrueckii subsp. bulgaricus under aerobic conditions as assessed by proteomic analysis

AccessionProtein descriptionMW [kDa]CoverageFold change
T/Ca
Cellular response to stimulus
 F0HVQ0SOS regulatory protein LexA22.162.912.228
 A0A061BVN8Regulatory protein RecX30.453.322.106
 S2KUP9Transcriptional regulator34.811.921.872
 A0A061BU68Transcriptional regulator (Crp family)35.633.431.714
 A0A061CJZ0Transcriptional regulator (MarR family)13.1317.391.636
 A0A061CK68Heat-inducible transcription repressor HrcA39.094.031.602
 A0A061BU38DNA repair protein RecO27.596.831.524
 Q1G937GroEL chaperonin57.3078.77− 1.592
 A0A061CKD6Chaperone protein DnaK66.0264.50− 1.600
 A0A061C1K9Response regulator27.8655.60− 1.715
 Q048Y2GroES chaperonin10.2785.11− 1.802
 A0A061BLC4Heat-shock protein Hsp2015.7956.03− 2.288
 G8I2M0Translation elongation factor Tu9.7134.44− 2.985
Carbohydrate transport and metabolism
 A0A061BX52EpsIIH, glycosyltransferase42.1618.011.802
 Q1G8F3EpsIM, glycosyltransferase40.5524.171.744
 Q1G8E9EpsIH, glycosyltransferase31.4828.091.624
 Q1G7Z6Pyruvate oxidase67.6764.941.592
 Q1G8E8EpsIG, glycosyltransferase19.1938.921.529
 Q1GAY6Acetate kinase43.1646.081.512
 Q71HT0Phosphopyruvate hydratase15.1582.19− 1.520
 A0A061BLT3Galactosyltransferase39.999.02− 1.567
 A0A061CQG7Phosphoglycerate kinase42.7180.89− 1.678
 D8FNY3Pyruvate kinase62.9053.99− 1.949
 A0A061BZB86-Phosphofructokinase38.7654.87− 2.268
 D8FN74d-lactate dehydrogenase36.9856.46− 2.283
 D8FM36Ribose-phosphate diphosphokinase36.9142.22− 3.636
Lipid transport and metabolism
 A0A061BKY2Acyl-phosphatelycerol 3-phosphate acyltransferase29.912.331.579
 A0A061BRJ8Glycerophosphoryl diester phosphodiesterase26.7316.961.500
 Q1G9C4Putative acylphosphatase10.0546.67− 1.522
 A0A061C1U5Geranylgeranyl pyrophosphate synthase32.9535.14− 1.667
 G6EWT6Cyclopropane-fatty-acyl-phospholipid synthase44.2244.16− 2.247
Amino acid transport and metabolism
 A0A061BW11Cysteine–tRNA ligase53.6537.97− 1.534
 D8FQC5Arginine–tRNA ligase64.1058.05− 1.610
 A0A061BMI1Homoserine O-succinyltransferase29.7913.85− 1.618
 Q1GBX1Amino acid ABC transporter, substrate binding protein31.9855.59− 1.626
 Q04CP9S-ribosylhomocysteine lyase 117.8568.55− 1.672
 A0A061BL94Amino acid ABC transporter, ATP-binding protein28.2552.44− 1.818
 A0A061BUC1Cystathionine gamma-synthase43.0644.67− 1.832
 G6EWC8Serine protease43.423.53− 1.873
 Q1G9F7Phosphoribosylformylglycinamidine synthase subunit PurQ24.2211.61− 2.165
 A0A061BUK8Cysteine synthase32.4358.31− 2.174
 A0A0D6ZH63Amino acid ABC transporter permease25.3412.95− 2.252
Genetic information processing
 Q1GBK050S ribosomal protein L306.6760.663.435
 Q04BZ950S ribosomal protein L1812.9273.952.171
 Q71J1750S ribosomal protein L2210.5868.372.147
 G6F488Ribosome maturation factor RimP15.5211.281.949
 D8FNZ8RNA polymerase sigma factor42.7630.421.760
 Q049M450S ribosomal protein L2111.3361.171.693
 Q1GBK430S ribosomal protein S814.4470.451.689
 A0A061CI0530S ribosomal protein S147.0911.481.687
 Q1G90450S ribosomal protein L1018.1870.411.647
 Q1GBL850S ribosomal protein L322.7573.681.598
 Q1GBI650S ribosomal protein L1316.3873.471.581
 F0HX4050S ribosomal protein L248.2158.231.572
 F0JZX630S ribosomal protein S1710.4945.451.542
 G6EVN150S ribosomal protein L421.8743.721.529
 Q04BZ250S ribosomal protein L364.4126.321.517
 Q1G7Z4tRNA modification GTPase MnmE49.9332.321.510
 Q1G90550S ribosomal protein L1212.3459.501.506
 F0HW2050S ribosomal protein L345.4113.04− 1.529
 Q1G8Z550S ribosomal protein L335.4920.41− 2.141
Nucleotide metabolism
 A0A061BNDCytidine deaminase15.434.322.065
 Q1GA92Pseudouridine synthase33.8711.221.955
 G6F8G6N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase15.5412.121.676
 F0JZF8Inosine-5′-monophosphate dehydrogenase40.1379.74− 1.534
 A0A061C7C2Putative pyridine nucleotide-disulphide oxidoreductase48.7059.69− 1.560
Other metabolism
 A0A0D6ZHP8Glutamine transporter, ATP-binding protein27.1610.161.714
 F0HUZ5FeS cluster assembly scaffold IscU15.8118.491.691
 Q1G9D0membrane protein93.971.681.635
 A0A061BP48Thioredoxin12.0079.251.542
 Q1GAT1Cell division protein SepF15.6362.321.534
 Q1GAK5Segregation and condensation protein B22.2139.001.528
 A0A061BY60Hydrolase (NUDIX family)19.838.62− 1.513
 A0A061BWU4ABC transporter, ATP-binding protein23.7753.30− 1.572
 Q1G869ATP-dependent Clp protease, ATP-binding subunit clpL76.9076.87− 1.600
 D8FM00Phosphonate ABC transporter, substrate-binding protein34.0846.33− 1.603
 D8FPT9ATP-dependent Clp protease, ATP-binding subunit clpE76.9661.78− 1.637
 G6EW12Peptide hydrolase51.3131.28− 1.650
 A0A061BU80Cation transporting P-type ATPase81.7129.80− 1.653
 A0A061BUM1Phosphoribosylglycinamide formyltransferase21.6410.88− 1.658
 A0A061BVN42,5-didehydrogluconate reductase31.0126.09− 1.678
 A0A061CDM4X-Pro dipeptidase PepQ41.0948.10− 1.783
 D8FLL0ATP synthase subunit beta52.1060.33− 1.832
 Q71IA33-Hydroxy-3-methylglutaryl-coenzyme A reductase15.9524.16− 1.852
 A0A061CI27ATP-dependent Clp protease, ATP-binding subunit clpC90.8348.60− 2.169
  1. aAverage fold-change was calculated as the ratio of the CcpA-negative mutant of L. bulgaricus ATCC11842 to the parental strain for up-regulated proteins and as the negative reciprocal values for down-regulated proteins, Proteins with the fold-change of ≥ 1.5 or ≤ − 1.5 and P-value < 0.05 were considered to be significantly up-regulated or down-regulated, respectively, based on statistic analysis for three biological replicates [19]