Table 1 Strains and plasmids used in this study
Strains and plasmids | Characteristics | Source or reference(s) |
|---|---|---|
Strains | ||
 E. coli DH5α | F−recA1 endA1 thi-1 supE44 relA1 deoR Δ(lacZYA-argF)U169Φ 80dlacZΔ M15 | Invitrogen |
 E. coli BL21(DE3) | F−ompT hsdSB(r−Bm−B) dcm gal λ(DE3) | Invitrogen |
 Comamonas sp. SWP-3 | Wild type, Strr, capable of degrading various herbicides | This study |
 Comamonas sp. SWP-3 M | The ppa gene-knockout mutant of strain SWP-3 with ppa gene, Strr | This study |
 Comamonas sp. SWP-3C | Mutant of strain SWP-3 with ppa gene restored, Strr | This study |
 Alicycliphilus sp. PH-34 | Wild type, Ampr, capable of degrading various aniline derivatives | This study |
Plasmids | ||
 pMD19-T | TA cloning vector; Ampr | TaKaRa |
 pEX18-Gm | Suicide vector; Gmr | TaKaRa |
 pBBR1MCS-2 | Broad host-range vector; kanamycinr (Kmr) | Lab store |
 pRK2013 | Conjugation helper plasmid | Lab store |
 pET-29a (+) | Expression vector; Kmr | Invitrogen |
 pMCS2-ppa | pBBR1MCS-2 containing the ppa gene; Kmr | This study |
 pET-ppa | pET-29a (+) containing the ppa gene; Kmr | This study |
 pEX18-ppa | pEX18-Gm containing the ppa gene; Gmr | This study |
 pMD19-ppa | pMD19-T containing the ppa gene; Ampr | This study |
 pMD19-ppa-T1 | pMD19-T containing the ppa-T1 gene; Ampr | This study |
 pMD19-ppa-T2 | pMD19-T containing the ppa-T2 gene; Ampr | This study |