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Table 1 Strains and plasmids used in this study

From: Mineralization of the herbicide swep by a two-strain consortium and characterization of a new amidase for hydrolyzing swep

Strains and plasmidsCharacteristicsSource or reference(s)
Strains
 E. coli DH5αFrecA1 endA1 thi-1 supE44 relA1 deoR Δ(lacZYA-argF)U169Φ 80dlacZΔ M15Invitrogen
 E. coli BL21(DE3)FompT hsdSB(rBmB) dcm gal λ(DE3)Invitrogen
 Comamonas sp. SWP-3Wild type, Strr, capable of degrading various herbicidesThis study
 Comamonas sp. SWP-3 MThe ppa gene-knockout mutant of strain SWP-3 with ppa gene, StrrThis study
 Comamonas sp. SWP-3CMutant of strain SWP-3 with ppa gene restored, StrrThis study
 Alicycliphilus sp. PH-34Wild type, Ampr, capable of degrading various aniline derivativesThis study
Plasmids
 pMD19-TTA cloning vector; AmprTaKaRa
 pEX18-GmSuicide vector; GmrTaKaRa
 pBBR1MCS-2Broad host-range vector; kanamycinr (Kmr)Lab store
 pRK2013Conjugation helper plasmidLab store
 pET-29a (+)Expression vector; KmrInvitrogen
 pMCS2-ppapBBR1MCS-2 containing the ppa gene; KmrThis study
 pET-ppapET-29a (+) containing the ppa gene; KmrThis study
 pEX18-ppapEX18-Gm containing the ppa gene; GmrThis study
 pMD19-ppapMD19-T containing the ppa gene; AmprThis study
 pMD19-ppa-T1pMD19-T containing the ppa-T1 gene; AmprThis study
 pMD19-ppa-T2pMD19-T containing the ppa-T2 gene; AmprThis study