Fig. 3

LC–MS analyses of the metabolite produced during swep degradation by recombinant Ppa purified from E. coli BL21 (DE3) (pET-ppa). The HPLC profiles of swep [Rt (min) = 6.497] and the metabolite 3,4-DCA [Rt (min) = 5.563] are indicated. The mass spectra of swep {m/z = 217.9780 [M − H]⁻} (right) and 3,4-DCA {m/z = 159.9722 [M − H]⁻} (left) are also shown