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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Molecular, structural and biochemical characterization of a novel recombinant chlorophyllase from cyanobacterium Oscillatoria acuminata PCC 6304

Fig. 3

a SDS-PAGE analysis of C-terminally 6 × His-tagged recombinant OaCLHs. M, prestained molecular weight marker. Lane 1, crude extract of recombinant full-length OaCLH. Lane 2, purified recombinant full-length OaCLH after Ni2+ affinity chromatography. Lane 3, crude extract of recombinant truncated OaCLH. Lane 4, purified recombinant truncated OaCLH after Ni2+ affinity chromatography. The protein gel was stained with Coomassie Brilliant Blue

b SDS-PAGE analysis of N-terminally 6 × His-tagged recombinant OaCLHs. M, prestained molecular weight marker. Lane 1, crude extract of recombinant full-length OaCLH. Lane 2, crude extract of recombinant truncated OaCLH. Lane 3, purified recombinant full-length OaCLH after Ni2+ affinity chromatography. Lane 4, purified recombinant truncated OaCLH after Ni2+ affinity chromatography. The protein gel was stained with Coomassie Brilliant Blue

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Microbial Cell Factories

ISSN: 1475-2859

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