Skip to main content
Fig. 5 | Microbial Cell Factories

Fig. 5

From: Highly selective synthesis of d-amino acids via stereoinversion of corresponding counterpart by an in vivo cascade cell factory

Fig. 5

Time course of d-Phe production for various concentrations of l-Phe, including a 50 mM, b 80 mM, c 100 mM, and d 150 mM. The concentration of E. coli pET-21b-MBP-laad/pET-28a-dapdh-fdh wet cells was 75 mg/mL, the dosage of NADP+ was 0.1 times the substrate concentration, and the dosage of ammonium formate was twice the substrate concentration. All reactions were carried out in Tris-HCl buffer (50 mM, pH 9.0) at 30 °C and 220 rpm. The values were averaged from triplicate measurements

Back to article page