Fig. 2
From: Production of biologically active human interleukin-10 by Bifidobacterium bifidum BGN4
a The structure of pBESIL10. b Restriction assay of pBES2 and pBESIL10. pBESIL10 is constructed with Pgap (gap promoter), the signal peptide of gene bbif_1761, hIL-10 (human Interleukin-10 ORF gene) optimized for Bifidobacterium bifidum, and terminator into pBES2. CmR and AmpR are chloramphenicol and ampicillin resistance genes; Ori is the origin of replication from pUC19; Mob and Rep are plasmid–encoded protein of MG1 from Bifidobacterium longum MG1 [38]. b Lane 1: 1 kb DNA ladder; Lane 2, 3, 4, 5: Xba1–Acc65I restriction of pBES2 shows one fragment (7.5 kb), as the other fragment is too small; Lane 6: Xba1–Acc65I restriction of pBESIL10 shows a 0.9 kb fragment (Insert fragment with Pgap, signal peptide, hIL-10 (optimized) and terminator connected, white arrow) with cut pBES2 (7.5 kb)