Table 1 PDCA titres (in mg/L) from (A) wild-type R. jostii RHA1 containing plasmid-expressed ligAB or praA genes [8]; (B) ΔpcaHG gene deletion strain containing plasmid-expressed ligAB or praA gene; (C) chromosomally expressed ligAB or praA genes under promoter control

A. Wild-type strain/plasmid

 R. jostii pTipQC2-ligAB

2,4-PDCA

168

112^a

90–125

 R. jostii pTipQC2-praA

2,5-PDCA

168

80^a

65–106

B. Gene deletion/plasmid

 R. jostii ΔpcaHG/pTipQC2-ligAB

2,4-PDCA

168

486^b

200

 R. jostii ΔpcaHG/pTipQC2-praA

2,5-PDCA

168

810^b

287

C. Chromosomal expression

 R. jostii ΔpcaHG:ligAB (no promoter)

2,4-PDCA

168

ND^b

ND

 R. jostii ΔpcaHG:ligAB(P_nitA)

2,4-PDCA

168

79

100

 R. jostii ΔpcaHG:ligAB(P_icl)

2,4-PDCA

168

ND

70

 R. jostii ΔpcaHG:ligAB(P_tpc5)

2,4-PDCA

168

290

164

 R. jostii ΔpcaHG:ligAB(P_tpc5)/pTipQC2-dyp2

2,4-PDCA

40

330

240