Fig. 5
2,4-PDCA production by R. jostii pcaHG:ligAB(Ptpc5) construct with or without expression of Amycolatopsis sp. 75iv2 dyp2 gene in 2.5 L bioreactor after 40 h, analysed by reverse phase HPLC. Maximum product titre was observed at 40 h for strain expressing dyp2, whereas strain lacking dyp2 showed maximum titre after 168 h (see Table 1). PCA, protocatechuic acid