Table 3 Characteristics of strains and plasmids used in the current study
Strains or plasmids | Relevant characteristic | Reference or source |
|---|---|---|
Strains | ||
Escherichia coli DH5α | Φ80 lacZΔM15 ΔlacU169 recA1 endA1 hsdR17 supE44 thi-1 gyrA relA1 | Yeast Collection Center of the Food Science and Technology Laboratory of Hainan University |
S. cerevisiae ABY3 | Industrial baker’s yeast | Yeast Collection Center of the Food Science and Technology Laboratory of Hainan University |
ABY+YP | Yep-PK | This study |
ABY+S | Yep-PSK | This study |
Plasmids | ||
pUG6 | E. coli/S. cerevisiae shuttle vector, containing Amp+, loxP-KanMX-loxP disruption cassette | Yeast Collection Center of the Food Science and Technology Laboratory of Hainan University |
Yep-P | URA3+, AmpR ori control vector, PGK1P-PGK1T | [18] |
Yep-PK | KanMX, PGK1P-PGK1T | |
Yep-PSK | KanMX, PGK1P-SNF1-PGK1T | |