Skip to main content

Table 4 Purification of VEGF165 from 6HFh8-VEGF165 fusion protein expressed in E. coli

From: Effective production of human growth factors in Escherichia coli by fusing with small protein 6HFh8

Purification step Concentration (mg/ml) Volume (ml) Total protein (mg) Fusion protein (tag-free protein) (mg) Purity (%)a Yield (%)b
Crude extract  1.5 ± 0.0 100 147.5 ± 0.7 40.8 ± 0.2 (18.8 ± 0.1) 27.1 100
HisTrap, 5 ml 0.3 ± 0.0 88 30.4 ± 0.6 29.0 ± 0.6 (13.4 ± 0.3) 95.9 79.8
HiTrap SP, 5 ml 0.11 ± 0.0 11 1.3 ± 0.0 NA (2.9 ± 0.1) 80.4 6.1
HisTrap, 5 ml 0.04 ± 0.0 21 0.8 ± 0.0 NA (2.8 ± 0.1) 95.8 4.6
HiTrap SP, 5 ml 0.03 ± 0.0 6 0.2 ± 0.0 NA (0.2 ± 0.0) > 99 0.9
  1. The data are shown as mean ± standard deviation from triplicate independent experiments
  2. aPurity was analyzed by densitometry using ImageJ and C18 RP HPLC
  3. bYield was calculated by dividing the tag-free protein of each purification product by tag-free protein in crude extract