Effective production of human growth factors in Escherichia coli by fusing with small protein 6HFh8

Table 3 Purification of aFGF from 6HFh8-aFGF fusion protein expressed in E. coli

Purification step	Concentration (mg/ml)^a	Volume (ml)	Total protein (mg)	Fusion protein (and tag-free) (mg)	Purity (%)^a	Yield (%)^b
Crude extract	4.6 ± 0.1	70.0	322.7 ± 8.9	153.0 ± 2.8 (81.0 ± 1.5)	47.4	100.0
HisTrap, 5 ml	2.5 ± 0.0	67.3	165.6 ± 2.9	142.5 ± 2.5 (75.5 ± 1.3)	86.1	93.2
HisTrap, 5 ml	1.6 ± 0.0	50.1	77.9 ± 2.5	NA (72.3 ± 2.3)	92.8	89.2
HiTrap CM, 5 ml	0.7 ± 0.0	80.5	59.6 ± 2.3	NA (59.6 ± 2.3)	100.0	73.5

The data are shown as mean ± standard deviation from triplicate independent experiments
^aPurity was analyzed by densitometry using ImageJ and C18 RP HPLC
^bYield was calculated by dividing the tag-free protein of each purification product by tag-free protein in crude extract

ISSN: 1475-2859