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Table 3 Purification of aFGF from 6HFh8-aFGF fusion protein expressed in E. coli

From: Effective production of human growth factors in Escherichia coli by fusing with small protein 6HFh8

Purification step Concentration (mg/ml)a Volume (ml) Total protein (mg) Fusion protein (and tag-free) (mg) Purity (%)a Yield (%)b
Crude extract  4.6 ± 0.1 70.0 322.7 ± 8.9 153.0 ± 2.8 (81.0 ± 1.5) 47.4 100.0
HisTrap, 5 ml 2.5 ± 0.0 67.3 165.6 ± 2.9 142.5 ± 2.5 (75.5 ± 1.3) 86.1 93.2
HisTrap, 5 ml 1.6 ± 0.0 50.1 77.9 ± 2.5 NA (72.3 ± 2.3) 92.8 89.2
HiTrap CM, 5 ml 0.7 ± 0.0 80.5 59.6 ± 2.3 NA (59.6 ± 2.3) 100.0 73.5
  1. The data are shown as mean ± standard deviation from triplicate independent experiments
  2. aPurity was analyzed by densitometry using ImageJ and C18 RP HPLC
  3. bYield was calculated by dividing the tag-free protein of each purification product by tag-free protein in crude extract