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Table 2 Production of 6HFh8-fused aFGF and VEGF165 using fed-batch fermentation

From: Effective production of human growth factors in Escherichia coli by fusing with small protein 6HFh8

GF End-point OD600 Dry cell weight (g/l) (wet cell) (g) Expression level (%)a Fusion protein (tag-free protein) (g/l) Solubility (%)
aFGF 126.8 ± 0.0 44.4 (254.6) 48.8 ± 0.6 9.7 (5.1) 98.2
VEGF165 73.8 ± 0.1 25.8 (150.2) 29.1 ± 0.3 3.4 (1.6) 100
  1. The data are shown as mean ± standard deviation from duplicate experiments
  2. aExpression level and solubility were analyzed by densitometry using ImageJ with duplicate experiments