Table 2 Production of 6HFh8-fused aFGF and VEGF165 using fed-batch fermentation
From: Effective production of human growth factors in Escherichia coli by fusing with small protein 6HFh8
GF | End-point OD600 | Dry cell weight (g/l) (wet cell) (g) | Expression level (%)a | Fusion protein (tag-free protein) (g/l) | Solubility (%) |
|---|---|---|---|---|---|
aFGF | 126.8 ± 0.0 | 44.4 (254.6) | 48.8 ± 0.6 | 9.7 (5.1) | 98.2 |
VEGF165 | 73.8 ± 0.1 | 25.8 (150.2) | 29.1 ± 0.3 | 3.4 (1.6) | 100 |