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Table 1 N-terminal sequence results of aFGF, EGF, IGF, and VEGF165. Expected sequence indicates the reference sequence, and observed sequence indicates the result of the N-terminal sequence. C was detected as X due to the characteristics of the Edman sequence method

From: Effective production of human growth factors in Escherichia coli by fusing with small protein 6HFh8

Protein

Sequence

Position

P1′

P2′

P3′

P4′

P5′

P6′

P7′

P8′

P9′

P10′

aFGF

Expected

F

N

L

P

P

G

N

Y

K

K

Observed

F

N

L

P

P

G

N

Y

K

K

EGF

Expected

N

S

D

S

E

C

P

L

S

H

Observed

N

S

D

S

E

X

P

L

S

H

IGF-1

Expected

G

P

E

T

L

C

G

A

E

L

Observed

G

P

E

T

L

X

G

A

E

L

VEGF165

Expected

A

P

M

A

E

G

G

G

Q

N

Observed

A

P

M

A

E

G

G

G

Q

N

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Microbial Cell Factories

ISSN: 1475-2859

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