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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Effective production of human growth factors in Escherichia coli by fusing with small protein 6HFh8

Fig. 6

Purification and analysis of VEGF165. a The overall purification steps. b SDS-PAGE analysis of samples from each purification step. c C18 RP-HPLC trace. d LC–MS (Q-TOF) analysis. M: marker; Lys: supernatant after sonication; 1: HisTrap purification; 2: after TEV protease treatment (the band at 28 kDa is TEVp); 3: HiTrap SP purification; 4: HisTrap purification; 5: HiTrap SP purification (final product). The data are representative of three replicated experiments

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