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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Construction and characterization of a novel glucose dehydrogenase-leucine dehydrogenase fusion enzyme for the biosynthesis of l-tert-leucine

Fig. 5

The synthesis of l-tle catalyzed by fusion enzyme or free enzyme system. The enzyme activities were kept as equal for both reaction systems. Reaction conditions: fusion enzyme or free enzyme system, 100 mM substrate, 0.4 mM NAD+, 30 °C, pH 9.0, 200 rpm. Yield means the ratio of the synthesized l-tle actually to the synthesized l-tle theoretically. Data represent the mean ± standard deviation of duplicate samples. Free enzyme system (green), fusion enzyme (blue)

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Microbial Cell Factories

ISSN: 1475-2859

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