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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Construction and characterization of a novel glucose dehydrogenase-leucine dehydrogenase fusion enzyme for the biosynthesis of l-tert-leucine

Fig. 4

Thermal stability of enzymes. a Free GDH and fusion GDH; b Free LeuDH and fusion LeuDH. Enzyme assays were performed in 0.8 M NH4Cl-NH3·H2O buffers (pH 10.0) or PBS buffers (pH 7.4) of at different pre-incubated temperatures for 1 h, using TMA and NADH as substrates (for LeuDH) or glucose and NAD+ as substrates (for GDH). Free enzyme (circles), fusion enzyme (triangles). Data represent the mean ± standard deviation of triplicate samples. Relative activity was based on enzyme activity before treatment as one hundred percent

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