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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Construction and characterization of a novel glucose dehydrogenase-leucine dehydrogenase fusion enzyme for the biosynthesis of l-tert-leucine

Fig. 3

Effects of pH and temperature on enzyme activities. a Effects of pH on GDH; b Effects of pH on LeuDH; c Effects of temperature on GDH; d Effects of temperature on LeuDH. Enzyme assays were performed in 0.8 M NH4Cl-NH3·H2O buffers or PBS buffers of different pH at 30 °C, using TMA and NADH as substrates (for LeuDH) or glucose and NAD+ as substrates (for GDH). Enzyme assays were performed in 0.8 M NH4Cl-NH3·H2O buffers (pH 10.0) or PBS buffers (pH 7.4) of at different temperatures, using TMA and NADH as substrates (for LeuDH) or glucose and NAD+ as substrates (for GDH). Free enzyme (circles), fusion enzyme (triangles). Data represent the mean ± standard deviation of triplicate samples. Relative activity was based on a percentage of maximum activity under the experimental conditions

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Microbial Cell Factories

ISSN: 1475-2859

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