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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Construction and characterization of a novel glucose dehydrogenase-leucine dehydrogenase fusion enzyme for the biosynthesis of l-tert-leucine

Fig. 2

The structure of recombinant E. coli. a Electrophoresis of PCR products and overlap PCR products of gdh and leudh (Lane: M, DNA marker; Lanes: 1, gdh; 2, leudh; 3, GDH–R3–LeuDH). b SDS-PAGE analysis of cell-free crude extracts of IPTG-induced recombinant E. coli (Lane: M, molecular weight marker; lane 1, control group; lane: 2, 4, 5, expression of GDH–R3–LeuDH fusion enzyme; lane: 3, expression of GDH; lane: 6, expression of LeuDH)

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Microbial Cell Factories

ISSN: 1475-2859

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