Table 1 Plasmids and strains used in this study
Plasmids and strains | Description | Source or reference |
|---|---|---|
Plasmids | ||
pRS306 | Integrative plasmid, URA3, Ampr | Laboratory stock |
pRS306-tHMGR1 | URA3, pRS306-PTPI1-tHMGR1-TCYC1 | This study |
pUG6 | loxp-KanMX-loxp, Ampr | Laboratory stock |
pRS425 | 2μ ori, LEU2, Ampr | Laboratory stock |
pRS425-NsGAS | LEU2, pRS425-PGAL1-NsGAS-TADH1 | This study |
pRS425-AmGAS | LEU2, pRS425-PGAL1-AmGAS-TADH1 | This study |
pRS425-ToGAS | LEU2, pRS425-PGAL1-ToGAS-TADH1 | This study |
pRS425-LsGAS | LEU2, pRS425-PGAL1-LsGAS-TADH1 | This study |
pRS425-AvGAS | LEU2, pRS425-PGAL1-AvGAS-TADH1 | This study |
pRS425-NcGAS | LEU2, pRS425-PGAL1-NcGAS-TADH1 | This study |
pRS425-CsGAS | LEU2, pRS425-PGAL1-CsGAS-TADH1 | This study |
pRS425-MmGAS | LEU2, pRS425-PGAL1-MmGAS-TADH1 | This study |
pRS425-SpGAS | LEU2, pRS425-PGAL1-SpGAS-TADH1 | This study |
pRS425-PsGAS | LEU2, pRS425-PGAL1-PsGAS-TADH1 | This study |
pRS425-AvGAS-L78A | LEU2, pRS425-PGAL1- AvGAS-L78A-TADH1 | This study |
pRS425- AvGAS-L78F | LEU2, pRS425-PGAL1- AvGAS-L78F-TADH1 | This study |
pRS425- AvGAS-L78T | LEU2, pRS425-PGAL1- AvGAS-L78T-TADH1 | This study |
pRS425-AvGAS-L78I | LEU2, pRS425-PGAL1- AvGAS-L78I-TADH1 | This study |
pRS425-AvGAS-L78G | LEU2, pRS425-PGAL1- AvGAS-L78G-TADH1 | This study |
pRS425-AvGAS-L78V | LEU2, pRS425-PGAL1- AvGAS-L78V-TADH1 | This study |
pRS425-AvGAS-A181C | LEU2, pRS425-PGAL1- AvGAS-A181C-TADH1 | This study |
pRS425-AvGAS-A181G | LEU2, pRS425-PGAL1- AvGAS-A181G-TADH1 | This study |
pRS425-AvGAS-A181F | LEU2, pRS425-PGAL1- AvGAS-A181F-TADH1 | This study |
pRS425-AvGAS-A181L | LEU2, pRS425-PGAL1- AvGAS-A181L-TADH1 | This study |
pRS425-AvGAS-A181Y | LEU2, pRS425-PGAL1- AvGAS-A181Y-TADH1 | This study |
pRS425-AvGAS-A181T | LEU2, pRS425-PGAL1- AvGAS-A181T-TADH1 | This study |
pRS425-AvGAS-A182T | LEU2, pRS425-PGAL1- AvGAS-A182T-TADH1 | This study |
pRS425-AvGAS-A182G | LEU2, pRS425-PGAL1- AvGAS-A182G-TADH1 | This study |
pRS425-AvGAS-A182S | LEU2, pRS425-PGAL1- AvGAS-A182S-TADH1 | This study |
pRS425-AvGAS-A182F | LEU2, pRS425-PGAL1- AvGAS-A182F-TADH1 | This study |
pRS425-AvGAS-A182L | LEU2, pRS425-PGAL1- AvGAS-A182L-TADH1 | This study |
pRS425-AvGAS-A183F | LEU2, pRS425-PGAL1- AvGAS-A183F-TADH1 | This study |
pRS425-AvGAS-A183S | LEU2, pRS425-PGAL1- AvGAS-A183S-TADH1 | This study |
pRS425-AvGAS-A183T | LEU2, pRS425-PGAL1- AvGAS-A183T-TADH1 | This study |
pRS425-AvGAS-A183G | LEU2, pRS425-PGAL1- AvGAS-A183G-TADH1 | This study |
pRS425-AvGAS-A183L | LEU2, pRS425-PGAL1- AvGAS-A183L-TADH1 | This study |
pRS425-AvGAS-L55F | LEU2, pRS425-PGAL1- AvGAS-L55F-TADH1 | This study |
RS425-AvGAS- L55W | LEU2, pRS425-PGAL1- AvGAS-L55W-TADH1 | This study |
pRS425-AvGAS- L55V | LEU2, pRS425-PGAL1- AvGAS-L55V-TADH1 | This study |
pRS425-AvGAS- L55A | LEU2, pRS425-PGAL1- AvGAS-L55A-TADH1 | This study |
pRS425-AvGAS- L55Y | LEU2, pRS425-PGAL1- AvGAS-L55Y-TADH1 | This study |
pRS425-AvGAS- I75A | LEU2, pRS425-PGAL1- AvGAS-I75A-TADH1 | This study |
pRS425-AvGAS- I75F | LEU2, pRS425-PGAL1- AvGAS-I75F-TADH1 | This study |
pRS425-AvGAS- I75L | LEU2, pRS425-PGAL1- AvGAS-I75L-TADH1 | This study |
pRS425-AvGAS- I75Y | LEU2, pRS425-PGAL1- AvGAS-I75Y-TADH1 | This study |
pRS425-AvGAS-F23W | LEU2, pRS425-PGAL1- AvGAS-F23W-TADH1 | This study |
pRS425-AvGAS-F23V | LEU2, pRS425-PGAL1- AvGAS-F23V-TADH1 | This study |
pRS425-AvGAS-F23Y | LEU2, pRS425-PGAL1- AvGAS-F23Y-TADH1 | This study |
pRS425-AvGAS-F23L | LEU2, pRS425-PGAL1- AvGAS-F23L-TADH1 | This study |
pRS425-AvGAS-H152Y | LEU2, pRS425-PGAL1- AvGAS-H152Y-TADH1 | This study |
pRS425-AvGAS-H152A | LEU2, pRS425-PGAL1- AvGAS-H152A-TADH1 | This study |
pRS425-AvGAS-H152G | LEU2, pRS425-PGAL1- AvGAS-H152G-TADH1 | This study |
pRS425-AvGAS-H152L | LEU2, pRS425-PGAL1- AvGAS-H152L-TADH1 | This study |
pRS425-AvGAS-K218R | LEU2, pRS425-PGAL1- AvGAS-K218R-TADH1 | This study |
pRS425-AvGAS-K218D | LEU2, pRS425-PGAL1- AvGAS-K218D-TADH1 | This study |
pRS425-AvGAS-K218E | LEU2, pRS425-PGAL1- AvGAS-K218E-TADH1 | This study |
Strains | ||
Saccharomyces cerevisiae CEN.PK2-1D | MATα; leu2-3,112; ura3-52; his3-Δ1; trp1-289; MAL2-8C; SUC2 | Laboratory stock |
SGAS1 | Expressing pRS425-AmGAS in CEN.PK2-1D | This study |
SGAS2 | Expressing pRS425-ToGAS in CEN.PK2-1D | This study |
SGAS3 | Expressing pRS425-LsGAS in CEN.PK2-1D | This study |
SGAS4 | Expressing pRS425-NsGAS in CEN.PK2-1D | This study |
SGAS5 | Expressing pRS425-AvGAS in CEN.PK2-1D | This study |
SGAS6 | Expressing pRS425-NcGAS in CEN.PK2-1D | This study |
SGAS7 | Expressing pRS425-CsGAS in CEN.PK2-1D | This study |
SGAS8 | Expressing pRS425-MmGAS in CEN.PK2-1D | This study |
SGAS9 | Expressing pRS425-SpGAS in CEN.PK2-1D | This study |
SGAS10 | Expressing pRS425-PsGAS in CEN.PK2-1D | This study |
SMVA1 | SGAS5; expressing pRS306-tHMGR1 | This study |
SMVA2 | SMVA1; PERG9Δ::loxP-PHXT1 | This study |
SMVA3 | SMVA2; DPP1Δ::loxP; LPP1Δ::loxP | This study |
SHEF | pRS306-tHMGR1; PERG9::loxP-PHXT1; DPP1Δ::loxP; LPP1Δ::loxP | This study |
SHEF-WT | Expressing pRS425-AvGAS in SHEF | This study |
SHEF-L78A | Expressing pRS425-AvGAS-L78A in SHEF | This study |
SHEF-L78F | Expressing pRS425-AvGAS-L78F in SHEF | This study |
SHEF-L78T | Expressing pRS425-AvGAS-L78T in SHEF | This study |
SHEF-L78I | Expressing pRS425-AvGAS-L78I in SHEF | This study |
SHEF-L78G | Expressing pRS425-AvGAS-L78G in SHEF | This study |
SHEF-L78V | Expressing pRS425-AvGAS-L78V in SHEF | This study |
SHEF-A181C | Expressing pRS425-AvGAS-A181C in SHEF | This study |
SHEF-A181G | Expressing pRS425-AvGAS-A181G in SHEF | This study |
SHEF-A181F | Expressing pRS425-AvGAS-A181F in SHEF | This study |
SHEF-A181L | Expressing pRS425-AvGAS-A181L in SHEF | This study |
SHEF-A181Y | Expressing pRS425-AvGAS-A181Y in SHEF | This study |
SHEF-A181T | Expressing pRS425-AvGAS-A181Y in SHEF | This study |
SHEF-A182T | Expressing pRS425-AvGAS-A182T in SHEF | This study |
SHEF-A182G | Expressing pRS425-AvGAS-A182G in SHEF | This study |
SHEF-A182S | Expressing pRS425-AvGAS-A182S in SHEF | This study |
SHEF-A182F | Expressing pRS425-AvGAS-A182F in SHEF | This study |
SHEF-A182L | Expressing pRS425-AvGAS-A182L in SHEF | This study |
SHEF-A183F | Expressing pRS425-AvGAS-A183F in SHEF | This study |
SHEF-A183S | Expressing pRS425-AvGAS-A183S in SHEF | This study |
SHEF-A183T | Expressing pRS425-AvGAS-A183T in SHEF | This study |
SHEF-A183G | Expressing pRS425-AvGAS-A183G in SHEF | This study |
SHEF-A183L | Expressing pRS425-AvGAS-A183G in SHEF | This study |
SHEF-L55F | Expressing pRS425-AvGAS-L55F in SHEF | This study |
SHEF-L55W | Expressing pRS425-AvGAS- L55W in SHEF | This study |
SHEF-L55V | Expressing pRS425-AvGAS-L55V in SHEF | This study |
SHEF-L55A | Expressing pRS425-AvGAS-L55A in SHEF | This study |
SHEF-L55Y | Expressing pRS425-AvGAS-L55Y in SHEF | This study |
SHEF- I75A | Expressing pRS425-AvGAS-I75A in SHEF | This study |
SHEF-I75F | Expressing pRS425-AvGAS-I75F in SHEF | This study |
SHEF-I75L | Expressing pRS425-AvGAS-I75L in SHEF | This study |
SHEF-I75Y | Expressing pRS425-AvGAS-I75Y in SHEF | This study |
SHEF-F23W | Expressing pRS425-AvGAS-F23W in SHEF | This study |
SHEF-F23V | Expressing pRS425-AvGAS-F23V in SHEF | This study |
SHEF-F23Y | Expressing pRS425-AvGAS-F23Y in SHEF | This study |
SHEF-F23L | Expressing pRS425-AvGAS-F23L in SHEF | This study |
SHEF-H152Y | Expressing pRS425-AvGAS-H152Y in SHEF | This study |
SHEF-H152A | Expressing pRS425-AvGAS-H152A in SHEF | This study |
SHEF-H152G | Expressing pRS425-AvGAS-H152G in SHEF | This study |
SHEF-H152L | Expressing pRS425-AvGAS-H152L in SHEF | This study |
SHEF-K218R | Expressing pRS425-AvGAS-K218R in SHEF | This study |
SHEF-K218D | Expressing pRS425-AvGAS-K218D in SHEF | This study |
SHEF-K218E | Expressing pRS425-AvGAS-K218E in SHEF | This study |