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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Improved production of germacrene A, a direct precursor of ß-elemene, in engineered Saccharomyces cerevisiae by expressing a cyanobacterial germacrene A synthase

Fig. 2

Phylogenetic analyses of the putative GASs used for enzyme screening and their orthologues. Previously known GASs were indicated by triangles. AvGAS identified in this study with the best performance on germacrene A production was indicated with an asterisk. The ten synthases applied for screening in this study were colored orange. The protein sequences of putative GASs or their orthologues were retrieved from NCBI or Uniprot databases with accession or entry numbers as follows: NsGAS (NCBI accession NO. WP_010998816), AmGAS (NCBI accession NO. AGD80135), ToGAS (NCBI accession NO. ALY05868), LsGAS (LTC2, NCBI accession NO. AAM11627), AvGAS (UniProt entry NO. Q3MBN2), NcGAS (UniProt entry NO. A0A1Z4HUB4), MmGAS (UniProt entry NO. V5BIL8), CsGAS (UniProt entry NO. A0A1Z4H7M3), SpGAS (UniProt entry NO. A0A1Y2N8F8), PsGAS (UniProt entry NO. A0A171DER6), NlGAS (NCBI accession NO. WP_190654468), MlGAS (NCBI accession NO. WP_024299200), SgGAS (NCBI accession NO. WP_190143472), SeGAS (NCBI accession NO. WP_060893906), PpGAS (NCBI accession NO. WP_189237389), NgPS (Pentalenene synthase, NCBI accession NO. SBO99268)

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Microbial Cell Factories

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