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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris

Fig. 5

Overview of the strain engineering strategies applied in this work and secretory pathway bottlenecks explored. Column graphs represent AppA phytase activity (mU/mL) after 132 h of cultivation. Strains names are abbreviated for simplicity: AppA (PAOX1) as PAOX1, AppA (PDAS1) as PDAS1, AppA (PCAT1) as PCAT1, AppA (PGAP) as PGAP, AppA (PHpFMD) as PHpFMD, AppA-HAC1 (PHpFMD-HpMOX) as HAC1, AppA-PDI (PHpFMD-HpMOX) as PDI, AppA-EUG1 (PHpFMD-HpMOX) as EUG1, AppA-MPDI (PHpFMD-HpMOX) as MPDI, AppA-ERV2 (PHpFMD-HpMOX) as ERV2, AppA-ERO1 (PHpFMD-HpMOX) as ERO1, AppA-SEC1 (PHpFMD-HpMOX) as SEC1, AppA-SLY1 (PHpFMD-HpMOX) as SLY1, AppA-PDI-KAR2 (PGAP) as PDI-KAR2, AppA-PDI-GPX1 (PCAT1) as PDI-GPX1, AppA-PDI-ERV2 (PGAP) as PDI-ERV2 and AppA-PDI-ERO1 (PGAP) as PDI-ERO1

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