Fig. 5From: Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastorisOverview of the strain engineering strategies applied in this work and secretory pathway bottlenecks explored. Column graphs represent AppA phytase activity (mU/mL) after 132Â h of cultivation. Strains names are abbreviated for simplicity: AppA (PAOX1) as PAOX1, AppA (PDAS1) as PDAS1, AppA (PCAT1) as PCAT1, AppA (PGAP) as PGAP, AppA (PHpFMD) as PHpFMD, AppA-HAC1 (PHpFMD-HpMOX) as HAC1, AppA-PDI (PHpFMD-HpMOX) as PDI, AppA-EUG1 (PHpFMD-HpMOX) as EUG1, AppA-MPDI (PHpFMD-HpMOX) as MPDI, AppA-ERV2 (PHpFMD-HpMOX) as ERV2, AppA-ERO1 (PHpFMD-HpMOX) as ERO1, AppA-SEC1 (PHpFMD-HpMOX) as SEC1, AppA-SLY1 (PHpFMD-HpMOX) as SLY1, AppA-PDI-KAR2 (PGAP) as PDI-KAR2, AppA-PDI-GPX1 (PCAT1) as PDI-GPX1, AppA-PDI-ERV2 (PGAP) as PDI-ERV2 and AppA-PDI-ERO1 (PGAP) as PDI-ERO1Back to article page