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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris

Fig. 4

ERO1, ERV2 and GPX1 co-expression further improves phytase yields while co-expression of HAC1 or KAR2 does not show improvement. a AppA co-expression with PDI and ERO1 or ERV2. b AppA co-expression with ERO1 or ERV2. c AppA co-expression with GPX1. d AppA co-expression with HAC1 or KAR2. Phytase production was determined by the p-NPP assay after methanol induction. The AppA-PDI (PHpFMD-HpMOX) strain was included for comparison. Data are represented as mean values ± standard deviation (n = 3)

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Microbial Cell Factories

ISSN: 1475-2859

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