Fig. 3From: Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastorisTranscriptional studies of AppA and PDI genes during phytase production. a Relative transcription of the AppA gene and b the PDI gene at 24, 48, 65, 66, 72 and 73 h. Methanol additions were performed at 65 and 72 h. ARG4 was used as reference gene. Data are represented as mean values ± standard deviation (n = 3)Back to article page