Table 1 Strains and plasmids used in this study

Strains

 E. coli BL21(DE3)

F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm rne131(DE3)

Invitrogen

 S. elongatus PCC 7942

Wild type

[17]

 Isoprene producer IP

BL21(DE3)/pYJM14 and pYJM20

[18]

Plasmids

 pYJM20

pACYCDuet-1 derivative carrying isoprene synthase gene ispS, HMG-CoA synthase gene mvaS and acetyl-CoA acetyltransferase/hydroxymethylglutaryl-CoA (HMG- CoA) reductase gene mvaE, T7 promoter, Cm^R

[18]

 pYJM14

pTrcHis2B derivative carrying mevalonate pyrophosphate decarboxylase gene ERG19, mevalonate kinase gene ERG12, phosphomevalonate kinase gene ERG8 and IPP isomerase gene IDI1, Trc promoter, Ap^R

[18]