Table 2 Description of the S. albus del14 engineered strains for heterologous expression of CHD BGC, and corresponding product yields
Introduced cosmid | ChdR | OxyD | OxyP | CHD BGC | Product | Yield |
|---|---|---|---|---|---|---|
pOJ456-CHD12 | ✓ | / | / | |||
pOJ436-CHD12 | ✓ |
| Up to 50 mg/L | |||
pOJ436-PermE*-chdR-CHD12 | ✓ | ✓ | Up to 60 mg/L | |||
pOJ436-PermE*-oxyDP-CHD12 | ✓ | ✓ | ✓ | / | / | |
pOJ436-PermE*-oxyDPchdR-CHD12 | ✓ | ✓ | ✓ | ✓ |
| ~ 3 mg/L |
