Fig. 5
(chromatograms adapted from DataAnalysis (Bruker Daltonics, Bremen))
LC–MS analysis of culture extracts of S. albus: Chromosomally-integrated empty cosmids pOJ436 (a) or pOJ436e*chdR (c) in comparison to culture extracts of S. albus containing chromosomally-integrated cosmids carrying the entire CHD BGC (pOJ436-CHD12) (b) or CHD BGC with additional copy of efflux pump chdR gene (pOJ436e*chdR-CHD12) (d). UV chromatograms at detection wavelength of 280 nm and EIC for m/z 412 (± 0.5), which corresponds to CHD, are shown