Fig. 1From: Translational landscape and protein biogenesis demands of the early secretory pathway in Komagataella phaffiiOverview of Ribo-seq and subcellular fractionation. Ribosomes (grey) bound to a translocon (red) are only solubilized in the presence of detergent. The total sample has footprints originating from both membrane-bound and free-floating ribosomes. The soluble fraction is enriched in footprints from free-floating ribosomes. The membrane fraction is enriched in footprints from membrane-bound ribosomesBack to article page