Fig. 1

GFP fluorescence, growth and product profile in control and double knockout (DKO) strains. Control and DKO strains were transformed with plasmids carrying genes for sfGFP and RubellaE1-sfGFP, and grown in TB media supplemented with 0.4% v/v glycerol and 10 mM MgSO₄. (a) GFP fluorescence profile (Excitation λ: 485 nm, Emission λ: 507 nm) in AU (arbitrary units) for control strain expressing sfGFP (orange) and Rubella E1-sfGFP (blue). b Growth profiles (biomass concentration measured in arbitrary units (AUs) by scattered light intensity at 620 nm) of control (blue) and DKO strain (red) containing pBAD-Rubella E1-sfGFP expression vector under induced (dotted lines) and uninduced (solid lines) conditions. c GFP fluorescence levels in control (blue) and DKO (red) strains expressing the Rubella sfGFP fusion protein. Data represents mean ± SD of three independent experiments