Table 2 Strains used in this study
From: Engineering of a genome-reduced strain Bacillus amyloliquefaciens for enhancing surfactin production
Strains | Relative characteristics | Source |
|---|---|---|
B. amyloliquefaciens | ||
 GR01 | LL3 derivative, Δupp | [24] |
 GR07 (NK-1) | GR01 ΔG0, 0.18% reduction of genome | [25] |
 GR22 | GR07 ΔG1, 0.55% reduction of genome | This work |
 GR46 | GR22 ΔG2, 1.15% reduction of genome | This work |
 GR94 | GR46 ΔG3, 2.36% reduction of genome | This work |
 GR134 | GR94 ΔG4, 3.36% reduction of genome | This work |
 GR164 | GR134 ΔG5, 4.11% reduction of genome | This work |
 GR167 | GR164 ΔG6, 4.18% reduction of genome | This work |
 NK-ΔLP | NK-1 derivative, ΔpgsBCA | [37] |
 GR167I | GR167 derivative, Δitu cluster | This work |
 GR167D | GR167 derivative, Δfen cluster | This work |
 GR167ID | GR167 derivative, Δitu Δfen clusters | This work |
 LL3-PRlac | LL3 derivative, containing plasmid pHT-PRlac-gfp | This work |
 LL3-PRugt | LL3 derivative, containing plasmid pHT-PRugt-gfp | This work |
 LL3-PRsuc | LL3 derivative, containing plasmid pHT-PRsuc-gfp | This work |
 LL3-PRydh | LL3 derivative, containing plasmid pHT-PRydh-gfp | This work |
 LL3-PRaccD | LL3 derivative, containing plasmid pHT-PRaccD-gfp | This work |
 LL3-PRclp | LL3 derivative, containing plasmid pHT-PRclp-gfp | This work |
 LL3-PRtpxi | LL3 derivative, containing plasmid pHT-PRtpxi-gfp | This work |
 LL3-PRgltX | LL3 derivative, containing plasmid pHT-PRgltX-gfp | This work |
 LL3-PRnad | LL3 derivative, containing plasmid pHT-PRnad-gfp | This work |
 LL3-PRarg | LL3 derivative, containing plasmid pHT-PRarg-gfp | This work |
 LL3-PRgltA | LL3 derivative, containing plasmid pHT-PRgltA-gfp | This work |
 LL3-PRahp | LL3 derivative, containing plasmid pHT-PRahp-gfp | This work |
 LL3-PRnrfA | LL3 derivative, containing plasmid pHT-PRnrfA-gfp | This work |
 LL3-PRpgmi | LL3 derivative, containing plasmid pHT-PRpgmi-gfp | This work |
 LL3-PRhom | LL3 derivative, containing plasmid pHT-PRhom-gfp | This work |
 LL3-PRhem | LL3 derivative, containing plasmid pHT-PRhem-gfp | This work |
 LL3-PRldh | LL3 derivative, containing plasmid pHT-PRldh-gfp | This work |
 LL3-PRrpsU | LL3 derivative, containing plasmid pHT-PRrpsU-gfp | This work |
 LL3-PRalsD | LL3 derivative, containing plasmid pHT-PRalsD-gfp | This work |
 GR167IDS | GR167ID derivative with its native srf promoter replaced by PRsuc | This work |
 GR167IDT | GR167ID derivative with its native srf promoter replaced by PRtpxi | This work |
 NK-ΔLPS | NK-ΔLP derivative with its native srf promoter replaced by PRsuc | This work |
E. coli strains | ||
 DH5α | supE44 ΔlacU169(_80 lacZΔM15) recA1 endA1 hsdR17(rK− mK+) thi-1gyrA relA1 F− Δ(lacZYA-argF) | Transgene |
 JM110 | F−dam-13::Tn9 (Camr) dcm-6 hsdR2 (rk−mk+) leuB6 hisG4 thi-1 araC14 lacY1 galK2 galT22 xylA5 mtl-1 rpsL136 (Strr) fhuA31 tsx-8 glnV44 mcrA mcrB1 | Fermentas |