Fig. 2

Characterization of dzl mutants. a Genomic PCR of the LCYE gene in the wild type and the ZEP, and dzl mutants. The intact LCYE gene yield a 752-bp PCR fragment. b DNA sequence alignment at the LCYE locus of the wild type, ZEP, and the dzl mutants. The 20-bp sequence before the PAM sequence (red) was used for in vitro sgRNA transcription. The inserted aph7 gene is shown in blue (full-length) and green (partial). Upside-down characters represent insertions in the reverse orientation. The right column indicates the length of the insertions at the target locus. c Southern blot analysis of dzl mutants. Genomic DNA (20 μg) of each strain was digested with either NcoI or PstI and probed with a PCR fragment corresponding to the aph7 gene. The pChlamy3 vector was used as a positive control